Journal: Cancer biology & therapy

Article Title: Tripartite motif-containing protein 50 suppresses triple-negative breast cancer progression by regulating the epithelial-mesenchymal transition.

doi: 10.1080/15384047.2024.2427410

Figure Lengend Snippet: Figure 4. Overexpressed TRIM50 eclipsed EMT biomarkers in TNBC. (a) Upper panel: western blot analysis of the indicated emt-associated proteins in MDA-MB 231, BT549, and Hs-578T cells with the indicated plasmids transfected. Lower panel: quantification of E-cadherin, N-cadherin, Vimentin, and SNAI1 levels relative to GAPDH in western blot. (b) Immunofluorescence analysis of SNAI1 in MDA-MB 231, BT549, and Hs-578T cells with the indicated plasmids transfected; n = 3 biological replicates. Scale bar 40 μm. The localization and fluorescence intensity of SNAI1 (green fluorescence) in the nucleus (blue fluorescence) were detected by immunofluorescence experiment after pcDNA3.1-TRIM50 and vector plasmid were transfected respectively. Scale bar: 20 µm. (c) Overexpression of TRIM50 inhibited tumorigenesis in vivo. Growth curves of tumorigenesis in nude mice were plotted and the final tumor mass was measured. Left panel: images of tumors on day 28 after injection. n = 6/ condition; right panel: tumor growth curves of tumorigenesis explicated in volume and weight at day 28 after injection of the above-described tumors. n = 6/condition. In scatter dot plots, each dot represents one mouse. Two-tailed student’s t-test was exploited.

Article Snippet: Rabbit polyclonal antibodies against SNAI1 (#26183-1-AP), E-cadherin (#20874-1-AP), N-cadherin (#22018-1-AP), vimentin (#10366-1-AP), GAPDH (#10494-1-AP) for WB were purchased from Proteintech Technology, USA.

Techniques: Western Blot, Transfection, Immunofluorescence, Fluorescence, Plasmid Preparation, Over Expression, In Vivo, Injection, Two Tailed Test